• Product NameNIT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NIT1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human nitrilase 1
• Target NameNIT1
• Alternative Names    MGC57670; nitrilase 1; Nitrilase homolog 1
• Accession No. Swiss-Prot#: Q86X76
  NCBI Gene ID: 4817
  Gene Accssion: BC046149/Q86X76
• UniprotQ86X76
• Gene ID4817;
• SDS-PAGE MW 36kd
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human placenta and fetal liver tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minute
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #36652 at dilution 1/50.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #36652 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting