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AGT Antibody

36739
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  Catalog No. Package Size Price
36739 100ul $279.00

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Description
  • Product Name AGT Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous levels of total AGT protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide corresponding to a region derived from internal residues of human angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • Target Name AGT
  • Alternative Names ANHU; SERPINA8
  • Accession No. Swiss-Prot#: P01019
    NCBI Gene ID: 183
    Gene Accssion: NP_000020
  • SDS-PAGE MW 53kd
  • Concentration 0.6mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:200-1:1000

Images
  • AGT Antibody - SAB | Signalway Antibody

    Gel: 10%SDS-PAGE
    Lysates (from left to right): Human liver cancer tissue
    Amount of lysate: 40ug per lane
    Primary antibody: 1/300 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 5 minutes

Background
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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