Product Detail
Product NameGSK3β(Phospho-Ser9) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.
Immunogen TypePeptide-KLH
Immunogen DescSynthesized phospho-peptide around the phosphorylation site of human GSK3β (phospho Ser9)
Target NameGSK3β
ConjugateUnconjugated
Other NamesFactor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta
Accession NoSwiss-Prot: P49841
NCBI Protein: NP_001139628.1
Uniprot
P49841
Gene ID
2932;
Sdspage MW46kd
Concentration1.0mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20°C for long term preservation (recommended). Avoid Store at 4°C for short term use.
Application Details
Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002 (left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β(Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002.
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #11002.
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3β (Phospho-Ser9) Antibody #11002.
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.
If you have published an article using product 11002, please notify us so that we can cite your literature.
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et al,Accumulation of 娄脗-catenin by lithium chloride in porcine myoblast cultures accelerates cell differentiation.
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et al,Signal regulatory protein 娄脕 negatively regulates mast-cell activation following Fc娄脜RI aggregation.
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et al,Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7.In Cell Death Dis.On 2018 Oct 22 by Yu B, Wu K et al..PMID: 30348980
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et al,Epithelial Cells Attenuate Toll-Like Receptor-Mediated Inflammatory Responses in Monocyte-Derived Macrophage-Like Cells to Mycobacterium tuberculosis by Modulating the PI3K/Akt/mTOR Signaling Pathway.In Mediators Inflamm.On 2018 Sep by Yang Y, Sun Y et al..PMID:30356420
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et al,Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats.In
Clin Exp Pharmacol Physiol on 2010 Oct by Tan WF, Cao XZ,et al..PMID:20659131
, (2010),
PMID: 20659131
et al,LIPUS Enhance Elongation of Neurites in Rat Cortical Neurons Through Inhibition of GSK-3beta.In Biomed Environ Sci on 2010 Jun by Cong Ren, Jia-Mou Li, et al..PMID:20708505
, (2010),
PMID: 20708505
et al,Quantitative proteomics reveals that miR-155 regulates the PI3K-AKT pathway in diffuse large B-cell lymphoma. In Am J Pathol on 2012 Jul by Huang X, Shen Y,et al..PMID:22609116
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PMID: 22609116
et al,Accumulation of 尾-Catenin by Lithium Chloride in Porcine Myoblast Cultures Accelerates Cell Differentiation.In Mol Biol Rep on 2011 Mar by Yingjuan Yang, Jinzeng Yang,et al..PMID:20857211
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PMID: 20857211
et al,Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. In Toxicol Lett on 2013 Mar 27 by Hongxia Zhang, Junjie Hou, et al..PMID: 23353032
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PMID: 23353032
et al,尾-N-methylamino-l-alanine causes neurological and pathological phenotypes mimicking Amyotrophic Lateral Sclerosis (ALS): The first step towards an experimental model for sporadic ALS. In Environ Toxicol Pharmacol on 2013 Sep by Estefan铆a de Munck, Emma Mu帽oz-S谩ez, et al..PMID: 23688553
, (2013),
PMID: 23688553
et al,Signal regulatory protein 伪 negatively regulates mast鈥恈ell activation following Fc蔚RI aggregation. In Eur J Immunol on 2013 Jun by Yu-fei Pan, Li-wei Dong, et al..PMID: 23504624
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PMID: 23504624
et al,Gastrokine-2 suppresses epithelial mesenchymal transition through PI3K/AKT/GSK3尾 signaling in gastric cancer.In Tumour Biol.On 2016 Sep by Dai J, Qian C et al..PMID:27323966
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et al, Overexpression of astrocyte-elevated gene-1 is associated with ovarian cancer development and progression.In Mol Med Rep on 2015 Apr by Bo Zhou , Jue Yang et al..PMID:25483832
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et al,Fumosorinone, a Novel PTP1B Inhibitor, Activates Insulin Signaling in Insulin-Resistance HepG2 Cells and Shows Anti-Diabetic Effect in Diabetic KKAy Mice .In Toxicol Appl Pharmacol On 2015 May 15 by Zhi-Qin Liu , Ting Liu et al..PMID: 25796170
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et al,A 尾1/2 Adrenergic Receptor鈥怱ensitive Intracellular Signaling Pathway Modulates CCL2 Production in Cultured Spinal Astrocytes.In J Cell Physiol on 2014 Mar by Norimitsu Morioka , Hiromi Abe et al..PMID:24037783
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et al,Nuclear factor E2-related factor 2 knockdown enhances glucose uptake and alters glucose metabolism in AML12 hepatocytes
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PMID: 28440735
et al,hERG activators exhibit antitumor effects in breast cancer through calcineurin and β-catenin-mediated signaling pathways.
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PMID: 39917621
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