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Elk-1(Phospho-Ser383) Antibody#11004

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Product Detail

Product NameElk-1(Phospho-Ser383) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Elk-1 only when phosphorylated at serine 383.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 383 (T-L-S(p)-P-I) derived from Human Elk-1.

Target NameElk-1

ConjugateUnconjugated

Other NamesELK1; ETS-domain protein Elk-1;

Accession NoSwiss-Prot: P19419
NCBI Protein: NP_001107595.1

Uniprot P19419

Gene ID 2002;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 47kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1(Phospho-Ser383) Antibody #11004.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1(Phospho-Ser383) Antibody #11004(left) or the same antibody preincubated with blocking peptide(right).
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.

Xing J, et al. (1996) Science. 273(5277): 959-963.
Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.
Marais R, et al. (1993) Cell 73: 381-393.
Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824.
Cavigelli M, et al. (1995) EMBO J. 14: 5957-5964.

If you have published an article using product 11004, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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