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Raf1(Phospho-Ser259) Antibody#11006

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Product NameRaf1(Phospho-Ser259) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.

Target NameRaf1

ConjugateUnconjugated

Other NamesC-RAF; C-Raf; CRAF

Accession NoSwiss-Prot: P04049
NCBI Protein: NP _002871.1

Uniprot P04049

Gene ID 5894;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 73kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 and MDA cells untreated or treated with PMA using Raf1(Phospho-Ser259) Antibody #11006.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Phospho-Ser259) Antibody #11006.

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.

If you have published an article using product 11006, please notify us so that we can cite your literature.

et al,Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer. , (2012),
PMID: 22738312
et al,Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.Incell Death Dis. On 2018 Feb 13 by Wang Z, Ma L et al..PMID:29440765 , (2018),
PMID: 29440765
et al,Prognostic Impact of Raf-1 and p-Raf-1 Expressions for Poor Survival Rate in Non-Small Cell Lung Cancer.In Cancer Sci on 2012 Oct by Zhi-Xin Qiu, Lei Wang,et al..PMID:22738312 , (2012),
PMID: 22738312
et al,Effect of Chaihushugan San on Expression of the Raf/mitogen-activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathway in the Hippocampi of Perimenopausal Rats Induced by Immobilization Stress .In J Tradit Chin Med on 2015 Aug by Li Shengqiang, Liang Wenna et al..PMID:26427116 , (2015),
PMID: 26427116
et al,Exosomes Derived From Human Mesenchymal Stem Cells Confer Drug Resistance in Gastric Cancer.In Cell Cycle on 2015 Aug 3 by Runbi Ji , Bin Zhang et al..PMID:26091251 , (2015),
PMID: 26091251
et al,Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway. In BMC Microbiol on 2016 Sep 23 by Yanan Li, Zhongjia Jiang,et al..PMID:27663303 , (2016),
PMID: 27663303
et al,MicroRNA-146b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury. In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu, et al..PMID: 27400799 , (2016),
PMID: 27400799

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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