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GSK3a(Phospho-Ser21) Antibody#11007

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Product Detail

Product NameGSK3a(Phospho-Ser21) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a.

Target NameGSK3a

ConjugateUnconjugated

Other NamesFactor A; GSK-3 alpha; kinase GSK3-alpha

Accession NoSwiss-Prot: P49840
NCBI Protein: NP_063937.2

Uniprot P49840

Gene ID 2931;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 51kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3a(Phospho-Ser21) Antibody #11007.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3a(Phospho-Ser21) Antibody #11007(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3a(Phospho-Ser21) Antibody #11007.
Western blot analysis of extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), using GSK3α (Phospho-Ser21) Antibody #11007.

Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.

Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.

If you have published an article using product 11007, please notify us so that we can cite your literature.

et al,Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway. , (2011),
PMID: 21861134
et al,Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway. In J Cancer Res Clin Oncol on 2011 Nov by Dan Liu, Yi Huang, et al..PMID:21861134 , (2011),
PMID: 21861134

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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