Product Detail
Product NameRel(Phospho-Ser503) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsIF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of Rel only when phosphorylated at serine 503.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 503 (T-S-S(p)-D-S) derived from Human Rel.
Target NameRel
ConjugateUnconjugated
Other NamesC-Rel
Accession NoSwiss-Prot: Q04864
NCBI Protein: NP_002899.1
Uniprot
Q04864
Gene ID
5966;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 78kd
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed Hela cells using Rel(Phospho-Ser503) Antibody #11020.
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 (PubMed 18037997)).
Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
Haskill, S. et al. (1991) Cell 65, 1281-9.
Thompson, J.E. et al. (1995) Cell 80, 573-82.
If you have published an article using product 11020, please notify us so that we can cite your literature.
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