Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > c-Jun(Phospho-Thr239) Antibody

c-Jun(Phospho-Thr239) Antibody#11024

Availability: Yes
Total Amount:$
Product Detail

Product Namec-Jun(Phospho-Thr239) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of c-Jun only when phosphorylated at threonine239.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 239 (G-E-T(p)-P-P) derived from Human c-Jun.

Target Namec-Jun

ConjugateUnconjugated

Other NamesAH119; AP1; Jun A; c-Jun; p39

Accession NoSwiss-Prot: P05412
NCBI Protein: NP_002219.1

Uniprot P05412

Gene ID 3725;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells untreated or treated with UV using c-Jun(Phospho-Thr239) Antibody #11024.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr239) Antibody #11024(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using c-Jun(Phospho-Thr239) Antibody #11024.
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Boyle W J, et al. (1991) Cell. 64(3): 573-584.
Binetruy B, et al. (1991) Nature. 351: 122-127.
Smeal T, et al. (1991) Nature. 354:494-496.
Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160.

If you have published an article using product 11024, please notify us so that we can cite your literature.

et al,Oncogenic Functions of Secreted Frizzled-Related Protein 2 in Human Renal Cancer. , (2010),
PMID: 20501806
et al,Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer. In Mol Cancer Ther on 2010 Jun by Soichiro Yamamura, Kazumori Kawakami, et al..PMID: 20501806 , (2010),
PMID: 20501806
et al,MFHAS1 Suppresses TLR4 Signaling Pathway via Induction of PP2A C Subunit Cytoplasm Translocation and Inhibition of c-Jun Dephosphorylation at Thr239.In Mol Immunol on 2017 Aug by Qiqing Shi , Bo Xiong,et al..PMID: 28609714 , (2017),
PMID: 28609714

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;