Product Detail
Product NameMyc(Phospho-Thr58) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Myc only when phosphorylated at threonine 58.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from Human Myc.
Target NameMyc
ConjugateUnconjugated
Other Namesc-myc
Accession NoSwiss-Prot: P01106
NCBI Protein: NP_002458.2
Uniprot
P01106
Gene ID
4609;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells using Myc(Phospho-Thr58) Antibody #11034(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Phospho-Thr58) Antibody #11034(left) or the same antibody preincubated with blocking peptide(right).
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Jin Z, et al. (2004) J Biol Chem. 279(38): 40209-40219.
Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101(24): 9085-9090.
Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
If you have published an article using product 11034, please notify us so that we can cite your literature.
et al,MYC protein dysregulation is driven by BCR鈥怭I 3K signalling in diffuse large B鈥恈ell lymphoma.In Histopathology on 2017 Nov by Wei-Ge Wang , Ze-Bing Liu,et al..PMID:28639315
, (2017),
PMID:
28639315
Yes
