Product Detail
Product NameSTAT1(Phospho-Tyr701) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from Human STAT1.
Target NameSTAT1
ConjugateUnconjugated
Other NamesCANDF7, ISGF-3, STAT91
Accession NoSwiss-Prot: P42224
NCBI Protein: NP _009330.1
Uniprot
P42224
Gene ID
6772;
Sdspage MW84,91kd
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 84,91kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
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If you have published an article using product 11044, please notify us so that we can cite your literature.
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et al,Danzhi Jiangtang Capsule ameliorates kidney injury via inhibition of the JAK-STAT signaling pathway and increased antioxidant capacity in STZ-induced diabetic nephropathy rats.In Biosci Trends.On 2019 Jan 22 by Sun M, Bu W et al..PMID: 30606979
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et al,Chronic Inflammation In Response to Injury: Retention of Myeloid Cells in Injured Tissue Is Driven by Myeloid Cell IntrInsic Factors. In J Invest Dermatol on 2019 Jan 28 by Torbica T, Wicks K, et al..PMID:30703358
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et al,The C-terminal Domain of Chikungunya Virus nsP2 Independently Governs Viral RNA Replication, Cytopathicity, and Inhibition of Interferon Signaling.In J Virol on 2013 Sep by Jelke J Fros, Erika van der Maten,et al..PMID:23864632
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et al,High-glucose Environment Reduces Human 尾-defensin-2 Expression in Human Keratinocytes: Implications for Poor Diabetic Wound Healing.In
Br J Dermatol on 2012 Jun by C-C E Lan, C-S Wu,et al..PMID:22283836
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PMID: 22283836
et al,Neural stem cells secrete factors that promote auditory cell proliferation via a leukemia inhibitory factor signaling pathway. In J Neurosci Res on 2010 Nov 15 by Hsin-Chien Chen, Hsin-I Ma, et al..PMID: 20882565
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PMID: 20882565
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20686047
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PMID: 20686047
et al,Inhibitory effects of chalcone glycosides isolated from Brassica rapa L. 鈥榟idabeni锟?and their synthetic derivatives on LPS-induced NO production in microglia. In Bioorg Med Chem on 2011 Sep 15 by Hirokazu Hara, Yoko Nakamura, et al..PMID:21856162
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et al,DJC suppresses Advanced Glycation End Products-Induced JAK-STAT signaling and ROS in mesangial cells.In Evid Based Complement Alternat Med on 2017 May 29 by Min Sun, Yan Li ,et al..PMID: 28630633
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et al,Inhibitory Effects of Chalcone Glycosides Isolated From Brassica Rapa L. 'Hidabeni' and Their Synthetic Derivatives on LPS-induced NO Production in Microglia.In Bioorg Med Chem on 2011 Sep 15 by Hirokazu Hara, Yoko Nakamura,et al..PMID: 21856162
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et al,Newly Synthesized 鈥楬idabeni锟?Chalcone Derivatives Potently Suppress LPS-Induced NO Production via Inhibition of STAT1, but Not NF-魏B, JNK, and p38, Pathways in Microglia.In Biol Pharm Bull On 2014 by Hirokazu Hara , Ryoko Ikeda et al..PMID: 24882415
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et al,Costunolide improved dextran sulfate sodium-induced acute ulcerative colitis in mice through NF-kB, STAT1/3, and Akt signaling pathways. In Int Immunopharmacol on 2020 Jul by Fan Xie, Hai Zhang, et al..PMID:32413737
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et al,Identification of Cytochrome P450 2E1 as a Novel Target in Neuroinflammation and Development of Its Inhibitor Q11 as a Treatment Strategy.
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