Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > STAT5a(Phospho-Ser780) Antibody

STAT5a(Phospho-Ser780) Antibody#11049

Availability: Yes
Total Amount:$
Product Detail

Product NameSTAT5a(Phospho-Ser780) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of STAT5A only when phosphorylated at serine 780.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 780 (R-L-S(p)-P-P) derived from Human STAT5A.

Target NameSTAT5a

ConjugateUnconjugated

Other NamesMGF; MPF; Mammary gland factor; STA5A,; STAT5

Accession NoSwiss-Prot: P42229
NCBI Protein: NP_003143.2

Uniprot P42229

Gene ID 6776;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from K562 cells untreated or treated with IL-4 using STAT5A(Phospho-Ser780) Antibody #11049.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5A(Phospho-Ser780) Antibody #11049(left) or the same antibody preincubated with blocking peptide(right).
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Gouilleux F, et al. (1994) EMBO J. 13: 4361-4369.
Dentelli P, et al. (1999) J Immunol. 163: 2151-2159.
Meinke A, et al. (1996) Mol Cell Biol. 16: 6937-6944.

If you have published an article using product 11049, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;