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STAT6(Phospho-Tyr641) Antibody#11050

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Product Detail

Product NameSTAT6(Phospho-Tyr641) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHuman,Rat,Mouse

SpecificityThe antibody detects endogenous level of STAT6 only when phosphorylated at tyrosine 641.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Tyrosine 641 (R-G-Y(p)-V-P) derived from Human STAT6.

Target NameSTAT6

ConjugateUnconjugated

Other NamesIL-4 Stat

Accession NoSwiss-Prot: P42226
NCBI Protein: NP_003144.3

Uniprot P42226

Gene ID 6778;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from Hela cells untreated or treated with IL-4 using STAT6(Phospho-Tyr641) Antibody #11050.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6(Phospho-Tyr641) Antibody #11050(left) or the same antibody preincubated with blocking peptide(right).

Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738.
Malabarba M G, et al. (1996) Biochem. J. 319:865-872.
Hou J, et al. (1994) Science. 265:1701-1706.
Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.
Takeda K, et al. (1996) Nature. 380: 627-630.

If you have published an article using product 11050, please notify us so that we can cite your literature.

et al,Novel Inhaled pan-JAK Inhibitor, LAS194046, reduces allergen-Induced airway Inflammation, late asthmatic response and pSTATs activation In Brown Norway rats. In J Pharmacol Exp Ther on 2019 May 13 by Calbet M, Ramis I, et al..PMID: 31085698 , (2019),
PMID: 31085698

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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