Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > CREB(Phospho-Ser133) Antibody

CREB(Phospho-Ser133) Antibody#11052

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(8) Other
Product Detail

Product NameCREB(Phospho-Ser133) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CREB only when phosphorylated at serine 133.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 133 (R-P-S(p)-Y-R) derived from Human CREB.

Target NameCREB

ConjugateUnconjugated

Other NamesCREB-1; CREB1;

Accession NoSwiss-Prot: P16220
NCBI Protein: NP _004370.1

Uniprot P16220

Gene ID 1385;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from Hela cells untreated or treated with UV using CREB(Phospho-Ser133) Antibody #11052.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser133) Antibody #11052(left) or the same antibody preincubated with blocking peptide(right).

CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .

Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al. (1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.

If you have published an article using product 11052, please notify us so that we can cite your literature.

et al,Gene expression pattern in PC12 cells with reduced PMCA2 or PMCA3 isoform: selective up-regulation of calmodulin and neuromodulin. In Mol Cell Biochem on 2012 Jan by Tomasz Boczek, Anna Kozaczuk, et al..PMID: 21912933 , (2012),
PMID: 21912933
et al,S-Equol Activates cAMP Signaling at the Plasma Membrane of INS-1 Pancreatic 尾-Cells and Protects Against Streptozotocin-Induced Hyperglycemia by Increasing 尾-Cell Function in Male Mice.In J Nutr on 2017 Sep by Hiroko Horiuchi, Atsuko Usami,et al..PMID: 28768836 , (2017),
PMID: 28768836
et al,Downregulation of microsomal glutathione-S-transferase 1 modulates protective mechanisms in differentiated PC12 cells.In J Physiol Biochem on 2014 Jun by Monika Sobczak , Tomasz Boczek et al ..PMID: 24419913 , (2014),
PMID: 24419913
et al,S-Equol enantioselectively activates cAMP-protein kinase A signaling and reduces alloxan-induced cell death in INS-1 pancreatic 尾-cells.In J Nutr Sci Vitaminol (Tokyo) on 2014 by Hiroko Horiuchi, Naoki Harada et al..PMID: 25297619 , (2014),
PMID: 25297619
et al,Mogrol Derived from Siraitia grosvenorii Mogrosides Suppresses 3T3-L1 Adipocyte Differentiation by Reducing cAMP-Response Element-Binding Protein Phosphorylation and Increasing AMP-Activated Protein Kinase Phosphorylation.In PLoS One on 2016 Sep 1 by Naoki Harada , Mikako Ishihara et al..PMID:27583359 , (2016),
PMID: 27583359
et al,Hydrogen sulfide decreases 尾-adrenergic agonist-stimulated lung liquid clearance by inhibiting ENaC-mediated transepithelial sodium absorption. In Am J Physiol Regul Integr Comp Physiol on 2015 Apr 1 by Alisa M Agn茅, Jan-Peter Baldin, et al..PMID: 25632025 , (2015),
PMID: 25632025
et al,Connexin Hemichannels Contribute to the Activation of cAMP Signaling Pathway and Renin Production. In Int J Mol Sci on 2020 Jun 23 by Jingru Hong, Jian Yao,et al..PMID:32585970 , (2020),
PMID: 32585970
More
et al,Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells. In BMC Complement Med Ther on 2022 Jun 25 by Weiyu Fu, Xuehua Liao, et al..PMID:35752787 , (2022),
PMID: 35752787

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors