Product Detail
Product NameIntegrin b3(Phospho-Tyr773) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Integrin b3 only when phosphorylated at tyrosine 773.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 773 (P-L-Y(p)-K-E) derived from Human Integrin b3.
Target NameIntegrin b3
ConjugateUnconjugated
Other NamesCD61 antigen; GP3A; GPIIIa; ITB3; Platelet membrane glycoprotein IIIa
Accession NoSwiss-Prot: P05106
NCBI Protein: NP_000203.2
Uniprot
P05106
Gene ID
3690;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells using Integrin b3(Phospho-Tyr773) Antibody #11060 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin b3(Phospho-Tyr773) Antibody #11060(left) or the same antibody preincubated with blocking peptide(right).
Integrin a-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin a-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins a-IIb/beta-3 and a-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin a-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin a-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Chandhoke SK, et al. (2004) J Cell Sci.117 (Pt 8): 1431-1441.
Datta A, et al. (2002) J Biol Chem.277 (6): 3943-3949.
Law DA, et al. (1996) J Biol Chem.271 (18): 10811-10815.
If you have published an article using product 11060, please notify us so that we can cite your literature.
et al,Targeting Tyrosine Phosphatases by 3-Bromopyruvate Overcomes Hyperactivation of Platelets from Gastrointestinal Cancer Patients
, (2019),
PMID:
31261776
Yes
