Product NameEzrin(Phospho-Tyr353) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.

Target NameEzrin

ConjugateUnconjugated

Other NamesCytovillin; EZRI; VIL2; Villin 2; p81

Accession NoSwiss-Prot: P15311
NCBI Protein: NP_001104547.1

Uniprot P15311

Gene ID 7430;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 81kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100