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Estrogen Receptor-a(Phospho-Ser106) Antibody#11071

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Product Detail

Product NameEstrogen Receptor-a(Phospho-Ser106) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Estrogen Receptor-a only when phosphorylated at serine 106.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine106 (S-P- S(p)-P-L) derived from Human Estrogen Receptor-a.

Target NameEstrogen Receptor-a

ConjugateUnconjugated

Other NamesER; ESR; ESR1; ESTR; ESTRA

Accession NoSwiss-Prot: P03372
NCBI Protein: NP_000116.2

Uniprot P03372

Gene ID 2099;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000.

Western Blot analysis of RAT-BRAIN cells using Phospho-ERα (S106) Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser106) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MCF7 cells, using Estrogen Receptor-alpha (Phospho-Ser106) Antibody. The lane on the left is blocked with the phospho peptide

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Ouyang X,et al. (1998)Carcinogenesis; 19(11): 2013-9.
Ouyang X,et al. (1998) Exp Cell Res; 241(2): 467-75.
Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.

If you have published an article using product 11071, please notify us so that we can cite your literature.

et al,Astragalus root induces ovarian β?oxidation and suppresses estrogen?dependent uterine proliferation.In Mol Med Rep. On 2018 Dec Orkhon B1, Kobayashi K et al..PMID: 30272268 , (2018),
PMID: 30272268

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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