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Estrogen Receptor-a(Phospho-Ser118) Antibody#11072

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Product Detail

Product NameEstrogen Receptor-a(Phospho-Ser118) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of Estrogen Receptor-aonly when phosphorylated at serine 118.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 118 (Q-L-S(p)-P-F) derived from Human Estrogen Receptor-a.

Target NameEstrogen Receptor-a

ConjugateUnconjugated

Other NamesER; ESR; ESR1; ESTR; ESTRA

Accession NoSwiss-Prot: P03372
NCBI Protein: NP_000116.2

Uniprot P03372

Gene ID 2099;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 66kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from MDA cells untreated or treated with Estradiol using Estrogen Receptor-a(Phospho-Ser118) Antibody #11072.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser118) Antibody #11072(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser118) Antibody #11072.
Western blot analysis of extracts from COS7 cells, treated with starve or calf intestinal phosphatase (CIP), using Estrogen Receptor-α (Phospho-Ser118) Antibody #11072.

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Ramsauer VP, et al. (2003)J Biol Chem; 278(32): 30142-7.
Jepson S, et al. (2002)Oncogene; 21(49): 7524-32.
Ren Z, et al. (2002)J Biol Chem; 277(41): 38486-93.
Eppenberger-Castori S, et al. (2001)J Clin Oncol; 19(3): 645-56.

If you have published an article using product 11072, please notify us so that we can cite your literature.

et al,KMT2C mediates the estrogen dependence of breast cancer through regulation of ERα enhancer function.In Oncogene.On 2018 May 14 by Gala K, Li Q et al..PMID: 29755131 , (2018),
PMID: 29755131
et al,ESR1 Ligand-Binding Domain Mutations in Hormone-Resistant Breast Cancer. In Nat Genet on 2013 Dec. In Nat Genet on 2013 Dec by Weiyi Toy , Yang Shen, et al..PMID:24185512 , (2013),
PMID: 24185512
et al,Mutation site and context dependent effects of ESR1 mutation in genome-edited breast cancer cell models.In Breast Cancer Res on 2017 May 23 by Amir Bahreini , Zheqi Li,et al..PMID: 28535794 , (2017),
PMID: 28535794
et al,Identification of DNA sequence variants in the estrogen receptor pathway in breast cancer. In Biochem Biophys Res Commun on 2005 Nov 4 by Zhaoyi Wang, Xintian Zhang,et al..PMID:16165085 , (2005),
PMID: 16165085

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;