Product Detail
Product NameEGFR(Phospho-Tyr1092) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1092.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine1092 (P-E-Y(p)-I-N) derived from Human EGFR.
Target NameEGFR
ConjugateUnconjugated
Other NamesERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
Gene ID
1956;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1092) Antibody #11081.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Tyr1092) Antibody #11081(left) or the same antibody preincubated with blocking peptide(right).
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Buerger C, et al. (2003) J Biol Chem; 278(39): 37610-21.
Wang XQ, (2003) J Biol Chem; 278(49): 48770-8.
Saito T, et al. (2004) Endocrinology; 145(9): 4232-43.
Pao W, et al. (2004) Proc Natl Acad Sci U S A; 101(36): 13306-11.
If you have published an article using product 11081, please notify us so that we can cite your literature.
et al,Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression. In Oncogenesis on 2015 Sep 7 by A Nukuda 1 , C Sasaki et al..PMID:26344692
, (2015),
PMID:
26344692
et al,The EGF/EGFR axis and its downstream signaling pathways regulate the motility and proliferation of cultured oral keratinocytesInFEBS Open BioOn2023 AugbyRyota Kobayashi, Emi Hoshikawa et al..PMID:37243482
, (2023),
PMID:
37243482
Yes
