Product Detail
Product NameCaveolin-1(Phospho-Tyr14) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Caveolin-1 only when phosphorylated at tyrosine 14.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 14 (H-L-Y(p)-T-V) derived from Human CAVEOLIN-1.
Target NameCaveolin-1
ConjugateUnconjugated
Other NamesCAV; CAV1;
Accession NoSwiss-Prot: Q03135
NCBI Protein: NP_001166366.1
Uniprot
Q03135
Gene ID
857;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 24kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with H2O2(lane 2) using Caveolin-1(Phospho-Tyr14) Antibody #11090.
Immunofluorescence staining of methanol-fixed Hela cells using Caveolin-1(Phospho-Tyr14) Antibody #11090.
The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (a and beta) are encoded by a single transcript from this gene.
Zhang Y, et al. (2005) Mol Cell Proteomics. 4(9): 1240-1250.
Labrecque L, et al. (2004) J Biol Chem. 279(50): 52132-52140.
Fielding PE, et al. (2004) Biochemistry. 43(9): 2578-2586.
Labrecque L, et al. (2003) Mol Biol Cell. 14(1): 334-347.
Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.
If you have published an article using product 11090, please notify us so that we can cite your literature.
et al,Albumin endocytosis via megalin in astrocytes is caveola- and Dab-1 dependent and is required for the synthesis of the neurotrophic factor oleic acid.
, (2009),
PMID:
19656258
et al,Podosomes in Migrating Microglia: Components and Matrix Degradation.In J Neuroinflammation on 2012 Aug 8 by Catherine Vincent, Tamjeed A Siddiqui,et al..PMID:22873355
, (2012),
PMID:
22873355
et al,Albumin endocytosis via megalin in astrocytes is caveola鈥恆nd Dab锟? dependent and is required for the synthesis of the neurotrophic factor oleic acid. In J Neurochem
on 2009 Oct by Andr茅 Bento-Abreu, Ana Velasco, et al..PMID:19656258
, (2009),
PMID:
19656258
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