Product Namep53(Phospho-Ser315) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of p53 only when phosphorylated at serine 315.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53.

Target Namep53

ConjugateUnconjugated

Other NamesTumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;

Accession NoSwiss-Prot: P04637
NCBI Protein: NP_000537.3

Uniprot P04637

Gene ID 7157;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 53kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200