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p90RSK(Phospho-Thr348) Antibody#11105

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Product Detail

Product Namep90RSK(Phospho-Thr348) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of p90RSK only when phosphorylated at threonine 348.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 348 (S-R-T(p)-P-R) derived from Mouse p90RSK

Target Namep90RSK

ConjugateUnconjugated

Other NamesKS6A1; MAPKAP-K1a; RPS6KA1; RSK1; S6K-alpha 1

Accession NoSwiss-Prot: P18653
NCBI Gene ID: 20111
NCBI Protein: NP_001006666.1

Uniprot P18653

Gene ID 20111;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Hela cells untreated or treated with UV using p90RSK(Phospho-Thr348) Antibody #11105.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK(Phospho-Thr348) Antibody #11105(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using p90RSK(Phospho-Thr348) Antibody #11105.

Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

Silverman E, et al. Mol Cell Biol. 2004 Dec; 24(24): 10573-10583.
Andrew D, et al. Biochem J. 2006 February 1; 393(Pt 3): 715

If you have published an article using product 11105, please notify us so that we can cite your literature.

et al,Inhibitory effects of resveratrol on hepatitis B virus X protein-induced hepatocellular carcinoma.In J Vet Sci on 2017 Dec 31 by Seungmo Park, Jihae Lim ,et al..PMID: 28385009 , (2017),
PMID: 28385009

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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