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Tau(Phospho-Ser235) Antibody#11106

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Product Detail

Product NameTau(Phospho-Ser235) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Tau only when phosphorylated at serine 235.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 235 (P-K-S(p)-P-S) derived from Human Tau.

Target NameTau

ConjugateUnconjugated

Other NamesMAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau

Accession NoSwiss-Prot: P10636
NCBI Protein: NP _001116538.1

Uniprot P10636

Gene ID 4137;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser235) Antibody #11106 and the same antibody preincubated with blocking peptide.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881.
Liu F, et al. (2002) FEBS Lett. 530(1-3): 209-214.
Sengupta A, et al. (1998) Arch Biochem Biophys. 357(2): 299-309.

If you have published an article using product 11106, please notify us so that we can cite your literature.

et al,The formation of tau pathological pathological phospho-epitopes in the axon is prevented by the dephosphorylation of selective sites in prinmary hippocampal neurons over-expressing human tau. , (2010),
PMID: 20550628
et al,The formation of tau pathological phospho鈥恊pitopes in the axon is prevented by the dephosphorylation of selective sites in primary hippocampal neurons over鈥恊xpressing human tau. In J Neurochem on 2010 Sep 1 by Johanne Bertrand, Patrick S茅n茅chal, et al..PMID: 20550628 , (2010),
PMID: 20550628

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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