Product Detail
Product NameTau(Phospho-Thr231) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Tau only when phosphorylated at threonine 231.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of threonine 231 (V-R-T(p)-P-P) derived from Human Tau.
Target NameTau
ConjugateUnconjugated
Other NamesMAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
Accession NoSwiss-Prot: P10636
NCBI Protein: NP _001116538.1
Uniprot
P10636
Gene ID
4137;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Yen S.H.et.al. (1997) Neurochem. 69:1709-1719.
Beausoleil S.A.et.al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135.
Wintjens R.et.al. (2001) Biol. Chem. 276:25150-25156.
Babu J.R.et.al.(2005) Neurochem. 94:192-203.
Goedert M., Spillantini M.G. (2000) Biochim. Biophys. Acta 1502:110-121.
If you have published an article using product 11110, please notify us so that we can cite your literature.
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