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Tau(Phospho-Ser404) Antibody#11112

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Product Detail

Product NameTau(Phospho-Ser404) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Tau only when phosphorylated at serine 404.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 404 (D-T-S(p)-P-R) derived from Human Tau.

Target NameTau

ConjugateUnconjugated

Other NamesMAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau

Accession NoSwiss-Prot: P10636
NCBI Protein: NP_001116538.1

Uniprot P10636

Gene ID 4137;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer
Immunofluorescence staining of methanol-fixed Hela cells using Tau(Phospho-Ser404) Antibody #11112.
Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Tau (Phospho-Ser404) Antibody #11112.
Western blot analysis of extracts from C6 cells using Tau(Phospho-Ser404) Antibody #11112.

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Li G, Yin H, et.al .(2004) J Biol Chem ; 279(16): 15938-45.
Noble W, et al. (2003) Neuron ; 38(4): 555-65.
Giasson BI, et al.(2002). Biochemistry; 41(51): 15376-87.
Lee G., et.al. (1989). Neuron 2:1615-1624.
Andreadis A.et.al. (1992) Biochemistry 31:10626-10633.

If you have published an article using product 11112, please notify us so that we can cite your literature.

et al,Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like Pathology and Cognitive Impairments.In J Alzheimers Dis.On 2018 by Mahaman YAR, Huang F et al..PMID:29710724 , (2018),
PMID: 29710724
et al,Trillium tschonoskii maxim extract attenuates abnormal Tau phosphorylation.Neural Regen Res.In Neural Regen Res.On 2018 May by Luo HB, Shang N. et al..PMID: 29863023 , (2018),
PMID: 29863023
et al,Evidence of altered depression and dementia-related proteins in the brains of young rats after ovariectomy. In J Neurochem. On 2018 Aug 8 by Fang YY1, Zeng P et al..PMID: 29939407 , (2018),
PMID: 29939407
et al,CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer s Disease.In Cell Rep. On 2018 Jul 17 by Shentu YP, Huo Y et al..PMID:30021167 , (2018),
PMID: 30021167
et al,Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats.In Aging (Albany NY). On 2018 Nov 12 by Salissou MTM, Mahaman YAR et al..PMID:30425189 , (2018),
PMID: 30425189
et al,Liraglutide Ameliorates HyperhomocysteInemia-Induced Alzheimer-Like Pathology and Memory Deficits in Rats via Multi-molecular TargetIng. In Neurosci Bull on 2019 Jan 10 by Zhang Y, Xie JZ, et al..PMID: 30632006 , (2019),
PMID: 30632006
et al,NeurotrophIn receptor p75 mediates amyloid β-Induced tau pathology. In Neurobiol Dis on 2019 Dec by Shen LL, Li WW, et al..PMID:31394202 , (2019),
PMID: 31394202
More
et al,Protection of melatonin against acidosis induced neuronal injuries.In J Cell Mol Med on 2020 May 4 by Shi Y, Cai EL, et al..PMID: 32364678 , (2020),
PMID: 32364678
et al,Tau-Induced Ca2+/Calmodulin-Dependent Protein Kinase-IV Activation Aggravates Nuclear Tau Hyperphosphorylation .In Neurosci Bull. On 2018 Apr by Wei YP, Ye JW et al..PMID: 28646348 , (2018),
PMID: 28646348
et al,Adiponectin Attenuates Streptozotocin-Induced Tau Hyperphosphorylation and Cognitive Deficits by Rescuing PI3K/Akt/GSK-3β Pathway. In Neurochem Res.On 2018 Feb by Xu ZP, Gan GS et al..PMID: 29098530 , (2018),
PMID: 29098530
et al,Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling.In J Neurochem on 2012 Jan by Chen LM, Xiong YS,et al..PMID:21496024 , (2012),
PMID: 21496024
et al,Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A). In J Biol Chem on 2012 Mar 30 by Xu-Ying Sun, Yu-Ping Wei, et al..PMID: 22334661 , (2012),
PMID: 22334661
et al,FLZ alleviates the memory deficits in transgenic mouse model of Alzheimer's disease via decreasing beta-amyloid production and tau hyperphosphorylation. In PLoS One on 2013 Nov 4 by Xiu-Qi Bao, Ning Li, et al..PMID: 24223757 , (2013),
PMID: 24223757
et al,Sex dimorphism profile of Alzheimer's disease-type pathologies in an APP/PS1 mouse model.In Neurotox Res on 2016 Feb by Shu-Sheng Jiao, Xian-Le Bu et al..PMID:26707129 , (2016),
PMID: 26707129
et al,Brain-derived neurotrophic factor protects against tau-related neurodegeneration of Alzheimer's disease.In Transl Psychiatry on 2016 Oct 4 by S-S Jiao , L-L Shen et al..PMID:27701410 , (2016),
PMID: 27701410
et al,Polyphyllin I attenuates cognitive impairments and reduces AD-like pathology through CIP2A-PP2A signaling pathway in 3XTg-AD mice. In FASEB J on 2020 Dec by Ying Zhou, Dichen Yang, et al..PMID:33070372 , (2020),
PMID: 33070372
et al,FoxO1 overexpression reduces Aβ production and tau phosphorylation in vitro. In Neurosci Lett on 2020 Nov 1 by Wei Zhang, Shanshan Bai,et al..PMID:32860886 , (2020),
PMID: 32860886
et al,Correcting abnormalities in miR-124/PTPN1 signaling rescues tau pathology in Alzheimer's disease. In J Neurochem on 2020 Aug by Tong-Yao Hou, Yang Zhou,et al..PMID:31951013 , (2020),
PMID: 31951013
et al,MAPT/Tau accumulation represses autophagy flux by disrupting IST1-regulated ESCRT-III complex formation: a vicious cycle in Alzheimer neurodegeneration. In Autophagy on 2020 Apr by Qiong Feng, Yu Luo, et al..PMID:31223056 , (2020),
PMID: 31223056
et al,A novel small-molecule PROTAC selectively promotes tau clearance to improve cognitive functions in Alzheimer-like models. In Theranostics on 2021 Mar 11 by Weijin Wang, Qiuzhi Zhou, et al..PMID:33859747 , (2021),
PMID: 33859747
et al,14-3-3ζ Captures SET in the Cytoplasm, Mediating Tau Pathology and Cognitive Impairments. , (2021),
PMID:
et al,A novel dephosphorylation targeting chimera selectively promoting tau removal in tauopathies. In Signal Transduct Target Ther on 2021 Jul 14 by Jie Zheng, Na Tian et al..PMID:34262014 , (2021),
PMID: 34262014
et al,HIF-1α Causes LCMT1/PP2A Deficiency and Mediates Tau Hyperphosphorylation and Cognitive Dysfunction during Chronic Hypoxia. In Int J Mol Sci on 2022 Dec 17 by Ling Lei, Jun Feng,et al..PMID:36555780 , (2022),
PMID: 36555780
et al,Gypenoside IX restores Akt/GSK-3β pathway and alleviates Alzheimer's disease-like neuropathology and cognitive deficitsInAging (Albany NY)On2023 Dec 12byLing Lei?1?2,?Yong Luo et al..PMID:?38095632 , (2023),
PMID: 38095632
et al,Physalin B reduces tau phosphorylation and cell apoptosis in HEK293 cells by activating FoxO1. In Curr Mol Pharmacol on 2024 by Wei Zhang, Yating Shi,et al..PMID:37489791 , (2024),
PMID: 37489791
et al,Correcting abnormalities in miR-124/PTPN1 signaling rescues tau pathology in Alzheimer's. In J Neurochem on 2020 Aug by Tong-Yao Hou, Yang Zhou,et al..PMID:31951013 , (2024),
PMID: 31951013
et al,Physalin B reduces tau phosphorylation and cell apoptosis in HEK293 cells by activating FoxO1. In Curr Mol Pharmacol on 2024 by Wei Zhang, Yating Shi,et al..PMID:37489791 , (2024),
PMID: 37489791
et al,Effectiveness and mechanisms of combined use of antioxidant nutrients in protecting against oxidative stress-induced neuronal loss and related neurological deficits. In CNS Neurosci Ther on 2024 Jul by Lu Wang, Yingjuan Wei,et al..PMID:39072940 , (2024),
PMID: 39072940
et al,Selective degradation of hyperphosphorylated tau by proteolysis-targeting chimeras ameliorates cognitive function in Alzheimer’s disease model mice. In Front Pharmacol on 2024 Jun 11 by Dongping Yao, Ting Li,et al..PMID:38919259 , (2024),
PMID: 38919259
et al,Correcting abnormalities in miR-124/PTPN1 signaling rescues tau pathology in Alzheimer's. In J Neurochem on 2020 Aug by Tong-Yao Hou, Yang Zhou,et al..PMID:31951013 , (2024),
PMID: 31951013
et al,PINK1 Alleviates Cognitive Impairments via Attenuating Pathological Tau Aggregation in a Mouse Model of Tauopathy , (2022),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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