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p90RSK(Phospho-Ser352) Antibody#11113

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Product Detail

Product Namep90RSK(Phospho-Ser352) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of p90RSK only when phosphorylated at serine 352.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 352 (R-D-S(p)-P-G) derived from Human p90RSK.

Target Namep90RSK

ConjugateUnconjugated

Other NamesKS6A1; MAPKAP-K1a; RPS6KA1; RSK-1; RSK1

Accession NoSwiss-Prot: Q15418
NCBI Protein: NP_001006666.1

Uniprot Q15418

Gene ID 6195;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 90kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HUVEC cells using p90RSK(Phospho-Ser352) Antibody #11113(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Western blot analysis of extracts from Hela cells, treated with PMA or calf intestinal phosphatase (CIP), using p90RSK (Phospho-Ser352) Antibody #11113.

Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

Silverman E, et al. Mol Cell Biol. 2004 Dec; 24(24): 10573-10583.
Itoh S, et al. J Biol Chem 2005 Jun 24; 280(25): 24135-24142
Eisenmann KM, et al. Cancer Res 2003 Dec 01; 63(23): 8330-7

If you have published an article using product 11113, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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