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Myosin Light Chain 2 (Phospho-Ser19) Antibody#11114

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Product Detail

Product NameMyosin Light Chain 2 (Phospho-Ser19) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Rt

SpecificityThe antibody detects endogenous level of Myosin Light Chain 2 only when phosphorylated at serine 19.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from Human Myosin Light Chain 2.

Target NameMyosin Light Chain 2

ConjugateUnconjugated

Other NamesLC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4

Accession NoSwiss-Prot: P24844

Uniprot P24844

Gene ID 10398;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myosin Light Chain 2 (Phospho-Ser19) Antibody #11114 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 293 cells untreated or treated with starvation using Myosin Light Chain 2 (Phospho-Ser19) Antibody #11114.

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion

Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619.
Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627
Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256

If you have published an article using product 11114, please notify us so that we can cite your literature.

et al,TLR-mediated secretion of endoplasmic reticulum aminopeptidase 1 from macrophages.In J Immunol on 2014 May 1 by Yoshikuni Goto, Kenji Ogawa et al..PMID:24688025 , (2014),
PMID: 24688025
et al, Phosphorylated myosin light chain 2 (p-MLC2) as a molecular marker of antemortem coronary artery spasm.In Med Sci Monit on 2016 Sep 19 by Liliang Li, Yuhua Li et al..PMID: 27643564 , (2016),
PMID: 27643564
et al,Tetramethylpyrazine Suppresses the Enhanced Ca2+ Sensitivity through Inhibiting the Expression of RhoA-ROCK in Artery of Simulated Weightlessness Rats. , (2022),
PMID:
et al,FOXD3 confers chemo-sensitivity in ovarian cancer through a miR-335/DAAM1/myosin II axis-dependent mechanismInJ Ovarian ResOn2023 Jan 10byShufen Wang?1,?Yan Ma? et al..PMID:?36627652 , (2023),
PMID: 36627652
et al,Gastrodin attenuates angiotensin II-induced vascular contraction and MLCK/p-MLC2 pathway activationIn Pharm BiolOn2023 DecbyZhi Guo , Xuan Yang et al..PMID:37211627 , (2023),
PMID: 37211627

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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