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Location： Home > Phospho Antibodies > FKHR(Phospho-Ser256) Antibody

FKHR(Phospho-Ser256) Antibody#11115

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Product NameFKHR(Phospho-Ser256) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

ApplicationsWB, IH, IF/IC, IP

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of FKHR only when phosphorylated at serine 256.

Immunogen TypePeptide-KLH

Immunogen DescA synthesized peptide derived from human FOXO1A around the phosphorylation site of Ser256.

Target NameFKHR

ConjugateUnconjugated

Other NamesFKHR; FOXO1;

Accession NoSwiss-Prot: Q12778
NCBI Protein: NP_002006.2

Uniprot Q12778

Gene ID 2308;

Calculated MW82kDa

Concentration1.0mg/ml

FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol,and 0.01% sodium azide

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1：500 - 1：1000), IHC 1：100 - 1：200, IF/IC 1：100 - 1：500, IP 1：10 - 1：100

Immunohistochemical analysis of FOXO1 (Phospho-S256)staining in human lung cancer formalin fixed paraffinembedded tissue section.
Immunofluorescent analysis of FOXO1 (Phospho-S256)staining in MCF7 cells.
Western blot analysis of FKHR(Phospho-Ser256) expression in 293T,293T Insulin,293T EGF,293T H202,HUVEC Insulin,HUVEC EGF,Mouse Brain,Mouse Lung,Rat Brain,Rat Heart.

If you have published an article using product 11115, please notify us so that we can cite your literature.

et al,Activation of AKT/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hipoxic pre-conditioning in adult rats. , (2010),
PMID: 20492357
et al,Activation of Akt/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats.In J Neurochem on 2010 Aug by Zhan L, Wang T, et al..PMID:20492357 , (2010),
PMID: 20492357
et al,Activation of Akt/FoxO and inactivation of MEK/ERK pathways contribute to induction of neuroprotection against transient global cerebral ischemia by delayed hypoxic postconditioning in adult rats.In Neuropharmacology on 2012 Oct by Zhan L, Li D,et al..PMID:22749925 , (2012),
PMID: 22749925
et al,Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K鈥揂kt signaling pathways. In Regul Pept on 2013 Feb 10 by Libing Ma, Jinxiu Li, et al..PMID: 23327998 , (2013),
PMID: 23327998
et al,Translocation of forkhead box O1 to the nuclear periphery induces histone modifications that regulate transcriptional repression of PCK1 in HepG2 cells.In Genes Cells on 2015 Apr by Tamaki Arai, Fumi Kano et al..PMID: 25736587 , (2015),
PMID: 25736587

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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