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b-Catenin(Phospho-Thr41/Ser45) Antibody#11116

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Product Detail

Product Nameb-Catenin(Phospho-Thr41/Ser45) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of b-Catenin only when phosphorylated at threonine 41/serine 45.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 41/serine 45 (A-T-T(p)-T-A-P-S(p)-L-S) derived from Human b-Catenin.

Target Nameb-Catenin

ConjugateUnconjugated

Other NamesCTNNB1; CATNB; CTNB1; CTNNB;

Accession NoSwiss-Prot: P35222
NCBI Protein: NP_001091679.1

Uniprot P35222

Gene ID 1499;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 92kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from SW626 cells using b-Catenin(Phospho-Thr41/Ser45) Antibody #11116(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Provost E, et al. (2003)J Biol Chem 22; 278(34): 31781-9.
Sakanaka C , et al. (2003)J Biochem (Tokyo); 132(5): 697-703.
Amit S, et al. (2002) Genes Dev; 16(9): 1066-76.
Liu C, et al. (2002) Cell; 108(6): 837-47.
Provost E, et al. (2005) Oncogene; 24(16): 2667-76.

If you have published an article using product 11116, please notify us so that we can cite your literature.

et al,Selective 14-3-3纬 induction quenches p-尾-catenin Ser37/Bax-enhanced cell death in cerebral cortical neurons during ischemia.In Cell Death Dis on 2014 Apr 17 by X J Lai, S Q Ye et al..PMID: 24743739 , (2014),
PMID: 24743739

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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