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cdc25C(Phospho-Ser216) Antibody#11118

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Product Detail

Product Namecdc25C(Phospho-Ser216) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C.

Target Namecdc25C

ConjugateUnconjugated

Other NamesCDC25M1; MPIP3;

Accession NoSwiss-Prot: P30307
NCBI Protein: NP_001781.2

Uniprot P30307

Gene ID 995;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 60 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with serum using cdc25C(Phospho-Ser216) Antibody #11118.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C(Phospho-Ser216) Antibody #11118(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using cdc25C(Phospho-Ser216) Antibody #11118.
cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348.
Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860.
Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677.
Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995.

If you have published an article using product 11118, please notify us so that we can cite your literature.

et al,Jaridonin-induced G2/M Phase Arrest in Human Esophageal Cancer Cells Is Caused by Reactive Oxygen Species-Dependent Cdc2-tyr15 Phosphorylation via ATM-Chk1/2-Cdc25C Pathway .In Toxicol Appl Pharmacol on 2015 Jan 15 by Yong-Cheng Ma , Nan Su et al..PMID:25450480 , (2015),
PMID: 25450480
et al,PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT. In Cell Death Dis on 2018 Apr 1 by Wen-Kai Shi, Xiao-Dong Zhu, et al..PMID: 29559632 , (2018),
PMID: 29559632

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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