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Androgen Receptor(Phospho-Ser650) Antibody#11120

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Product Detail

Product NameAndrogen Receptor(Phospho-Ser650) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of AndrogenReceptor only when phosphorylated at serine 650.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 650 (T-T-S(p)-P-T) derived from Human Androgen Receptor.

Target NameAndrogen Receptor

ConjugateUnconjugated

Other NamesANDR; DHTR; AR

Accession NoSwiss-Prot: P10275
NCBI Protein: NP_000035.2

Uniprot P10275

Gene ID 367;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 110kd
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Androgen Receptor (Phospho-Ser650) Antibody #11120 (left) or the same antibody preincubated with blocking peptide #51120 (right).
Western blot analysis of extracts from 293 cells, treated with PMA or calf intestinal phosphatase (CIP), using Androgen Receptor (Phospho-Ser650) Antibody #11120.

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Brinkman, A.O. et al. (1999) J. Steroid. Biochem. Mol. Biol. 69, 307-313.
Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

If you have published an article using product 11120, please notify us so that we can cite your literature.

et al,Androgen receptor serine 81 mediates Pin1 interaction and activity. In Cell Cycle on 2012 Sep 15 by Raffaele La Montagna, Isabella Caligiuri, et al..PMID:22894932 , (2012),
PMID: 22894932

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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