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ATM(Phospho-Ser1981) Antibody#11122

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Product NameATM(Phospho-Ser1981) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of ATM only when phosphorylated at serine 1981.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.

Target NameATM

ConjugateUnconjugated

Other NamesAtaxia telangiectasia mutated homolog; Ataxia telangiectasia mutated; kinase ATM

Accession NoSwiss-Prot: Q13315
NCBI Protein: NP_000042.3

Uniprot Q13315

Gene ID 472;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 350kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Western blot analysis of extracts from Hela cells untreated or treated with UV using ATM(Phospho-Ser1981) Antibody #11122
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM(Phospho-Ser1981) Antibody #11122(left) or the same antibody preincubated with blocking peptide(right).

ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

Gupta A. et al. (2005) Mol Cell Biol. 25(12): 5292-5305.
Bernstein JL. et al. (2002) Breast Cancer Res. 4(6): 249-252.
Silverman J. et al. (2004) Genes Dev. 18(17): 2108-2119.
Nakada D. et al. (2003) Nucleic Acids Res. 31(6): 1715-1724.
 

If you have published an article using product 11122, please notify us so that we can cite your literature.

et al,Expression status of ataxia telangiectasia mutated gene coorelated with Prognosis in advanced gastric cancer , (2008),
PMID: 17928013
et al,Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2-ATM pathway in telomerase-overexpressing primary fibroblasts. , (2011),
PMID: 21824286
et al,Identification of Signaling Pathways Mediating Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis in Human Trophoblasts, Infect. , (2012),
PMID: 22689813
et al,ATM localization and gene expression in the adult mouse eye. , (2009),
PMID: 19234633
et al,ATM localization and gene expression in the adult mouse eye. In Mol Vis on 2009 by Julia Leemput, Christel Masson, et al..PMID:19234633 , (2009),
PMID: 19234633
et al,Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2鈥怉TM pathway in telomerase鈥恛verexpressing primary fibroblasts. In FEBS J on 2011 Oct by Ling L Tay, Wei H Tan, et al..PMID: 21824286 , (2011),
PMID: 21824286

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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