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Location： Home > Phospho Antibodies > FAK(Phospho-Tyr925) Antibody

FAK(Phospho-Tyr925) Antibody#11123

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Product Detail

Product NameFAK(Phospho-Tyr925) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 925.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 925 (K-V-Y(p)-E-N) derived from Human FAK.

Target NameFAK

ConjugateUnconjugated

Other NamesFADK 1; FAK1; PTK2

Accession NoSwiss-Prot: Q05397
NCBI Protein: NP_005598.3

Uniprot Q05397

Gene ID 5747;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells using FAK(Phospho-Tyr925) Antibody #11123(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunofluorescence staining of methanol-fixed Hela cells using FAK(Phospho-Tyr925) Antibody #11123.
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.
Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.
Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.

If you have published an article using product 11123, please notify us so that we can cite your literature.

et al,Cytotoxic activity of Shp2 inhibitor fumosorinone in human cancer cells.In Oncol Lett.On 2018 Jun by Chen C, Xue T et al..PMID:29928374 , (2018),
PMID: 29928374
et al,Human mesenchymal stromal cells in adhesion to cell-derived extracellular matrix and titanium: Comparative kInome profile analysis. In J Cell Physiol on 2019 Mar by Baroncelli M, Fuhler GM, et al..PMID:30058720 , (2019),
PMID: 30058720
et al,Platelet-dependent signaling and Low Molecular Weight Protein Tyrosine Phosphatase expression promote aggressive phenotypic changes in gastrointestinal cancer cells. In Biochim Biophys Acta Mol Basis Dis on 2022 Jan 1 by Alessandra V S Faria, Bingting Yu, et al..PMID: 34610471 , (2022),
PMID: 34610471
et al,PAK1-Dependent Regulation of Microtubule Organization and Spindle Migration Is Essential for the Metaphase I–Metaphase II Transition in Porcine Oocytes In Biomolecules on 2024 Feb 17 by Lei Peng, Yijing He,et al..PMID:38397472 , (2024),
PMID: 38397472
et al,Targeting Tyrosine Phosphatases by 3-Bromopyruvate Overcomes Hyperactivation of Platelets from Gastrointestinal Cancer Patients , (2019),
PMID: 31261776
et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer , (2021),
PMID: 34830928

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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