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LIMK1(Phospho-Thr508) Antibody#11126

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Product Detail

Product NameLIMK1(Phospho-Thr508) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1.

Target NameLIMK1

ConjugateUnconjugated

Other NamesLIMK-1; kinase LIMK1;

Accession NoSwiss-Prot: P53667
NCBI Protein: NP_002305.1

Uniprot P53667

Gene ID 3984;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000.

Western Blot analysis of HEPG2 using Phospho-LIMK-1 (T508) Polyclonal Antibody. Antibody was diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using LIMK1 (Phospho-Thr508) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COLO205 cells, using LIMK1 (Phospho-Thr508) Antibody. The lane on the right is blocked with the phospho peptide.

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cog

If you have published an article using product 11126, please notify us so that we can cite your literature.

et al,DGCR6L, A Novel PAK4 Interaction Protein, Regulates PAK4-mediated migration of Human Gastric Cancer Cell via LIMK1. , (2008),
PMID: 19778628
et al,DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1. In Int J Biochem Cell Biol on 2010 Jan by Xiaodong Li, Qiang Ke, et al..PMID: 19778628 , (2010),
PMID: 19778628

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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