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HDAC8(Phospho-Ser39) Antibody#11128

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Product Detail

Product NameHDAC8(Phospho-Ser39) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of HDAC8 only when phosphorylated at serine 39.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 39 (R-A-S(p)-M-V) derived from Human HDAC8.

Target NameHDAC8

ConjugateUnconjugated

Other NamesHD8; HDA8; HDACL1

Accession NoSwiss-Prot: Q9BY41
NCBI Protein: NP_001159890.1

Uniprot Q9BY41

Gene ID 55869;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 42kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8(Phospho-Ser39) Antibody #11128(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using HDAC8(Phospho-Ser39) Antibody #11128.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC8 gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

Lee H, et al. (2004) Mol Cell Biol. 24(2): 765-773.

If you have published an article using product 11128, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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