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IKK a(Phospho-Thr23) Antibody#11129

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Product Detail

Product NameIKK a(Phospho-Thr23) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of IKKa only when phosphorylated at threonine 23.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 23 (L-G-T(p)-G-G) derived from Human IKK a.

Target NameIKK a

ConjugateUnconjugated

Other NamesI kappa-B kinase alpha; I-kappa-B kinase 1; IKK-A; IKK-alpha; IKK1

Accession NoSwiss-Prot: O15111
NCBI Protein: NP_001269.3

Uniprot O15111

Gene ID 1147;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 85kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23) Antibody #11129.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Phospho-Thr23) Antibody #11129(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK α (Phospho-Thr23) Antibody #11129.

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Yuan ZQ, et al.(2002) J Biol Chem; 277(33): 29973-82.
Ozes ON, et al. (1999) Nature; 401(6748): 82-5.

If you have published an article using product 11129, please notify us so that we can cite your literature.

et al,Noncanonical NF-魏B activation mediates STAT3-stimulated IDO upregulation in myeloid-derived suppressor cells in breast cancer.In J Immunol on 2014 Sep 1 by Jinpu Yu , Yue Wang et al..PMID:25063873 , (2014),
PMID: 25063873
et al,Protective effects of chlorogenic acid on inflammatory responses induced by Staphylococcus aureus and milk protein synthesis in bovine mammary epithelial cells. In Microb Pathog on 2022 Oct by Qiang Ji, Meng Zhang, et al..PMID: 35995255 , (2022),
PMID: 35995255

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;