Product Detail
Product NameRb(Phospho-Ser795) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of Rb only when phosphorylated at serine 795.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 795 (P-S-S(p)-P-L) derived from Human Rb.
Target NameRb
ConjugateUnconjugated
Other NamesP105-RB; PP105; PP110; RB-1; RB1
Accession NoSwiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
Gene ID
5925;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with serum(lane 2) using Rb(Phospho-Ser795) Antibody #11130.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser795) Antibody #11130(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Rb(Phospho-Ser795) Antibody #11130.
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Fang Sun , Hanjiang Fu , Qin Liu, et al. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest. FEBS Letters, 582:1564
If you have published an article using product 11130, please notify us so that we can cite your literature.
et al,Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest.
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et al,T-helper-1-cell Cytokines Drive Cancer Into Senescence. In Nature on 2013 Feb 21 by Heidi Braum眉ller, Thomas Wieder, et al..PMID: 23376950
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et al,EEN regulates the proliferation and survival of multiple myeloma cells by potentiating IGF-1 secretion.In Biochem Biophys Res Commun on 2014 May 2 by Er-Wen Huang , Sheng-Jiang Xue et al ..PMID: 24704450
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et al, Nuclear distribution of claudin-2 increases cell proliferation in human lung adenocarcinoma cells.In Biochim Biophys Acta on 2014 Sep by Akira Ikari, Ryo Watanabe et al..PMID: 24907662
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PMID:
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