Product Detail
Product NameRb(Phospho-Ser807) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Rb only when phosphorylated at serine 807.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 807 (Y-I-S(p)-P-L) derived from Human Rb.
Target NameRb
ConjugateUnconjugated
Other NamesP105-RB; PP105; PP110; RB-1; RB1
Accession NoSwiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
Gene ID
5925;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser807) Antibody #11131.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser807) Antibody #11131(left) or the same antibody preincubated with blocking peptide(right).
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.
Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.
Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.
Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.
If you have published an article using product 11131, please notify us so that we can cite your literature.
et al,miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes.
, (2008),
PMID:
18701644
et al,The reversal effect of Ginsenoside Rh2 on drug resistance in human colorectal carcinoma cells and its mechanism. In Hum Cell.On 2018 Jul by Liu GW, Liu YH et al..PMID:29582366
, (2018),
PMID:
29582366
et al,Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells.In Toxicol In Vitro on 2011 Oct by
Cai X, Ye T,et al..PMID:21601631
, (2011),
PMID:
21601631
et al,Vinpocetine Inhibits Breast Cancer Cells Growth in Vitro and in Vivo.In Apoptosis on 2012 Oct by Er-Wen Huang, Sheng-Jiang Xue, et al..PMID:22729609
, (2012),
PMID:
22729609
et al,miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes. In Nucleic Acids Res on 2008 Sep by Qin Liu, Hanjiang Fu, et al..PMID: 18701644
, (2008),
PMID:
18701644
Yes
