Product NameASK1(Phospho-Ser83) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of ASK1 only when phosphorylated at serine 83.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 83 (G-S-S(p)-V-G) derived from Human ASK1.

Target NameASK1

ConjugateUnconjugated

Other NamesASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5

Accession NoSwiss-Prot: Q99683
NCBI Protein: NP_005914.1

Uniprot Q99683

Gene ID 4217;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100