Product Detail
Product NameIRS-1(Phospho-Ser636) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total IRS-1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 636 (P-M-S(p)-P-K) derived from Human IRS-1.
Target NameIRS-1
ConjugateUnconjugated
Other NamesIRS-1; IRS1;
Accession NoSwiss-Prot: P35568
NCBI Protein: NP_005535.1
Uniprot
P35568
Gene ID
3667;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 180kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 3T3 cells untreated or treated with Insulin using IRS-1(Phospho-Ser636) Antibody #11230.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1(Phospho-Ser636) Antibody #11230(left) or the same antibody preincubated with blocking peptide(right).
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
If you have published an article using product 11230, please notify us so that we can cite your literature.
et al,The role of mitochondrial oxidative stress in the metabolic alterations in diet-induced obesity in rats. In FASEB J on 2019 Nov by Mar n-Royo G, Rodr guez C, et al..PMID:31370681
, (2019),
PMID:
31370681
et al,Metabolic inflammation exacerbates dopaminergic neuronal degeneration in response to acute MPTP challenge in type 2 diabetes mice.In Exp Neurol.On 2014 Jan by Wang L, Zhai YQ et al..PMID:24220636
, (2014),
PMID:
24220636
Yes
