Product Detail
Product NamePKCd(Phospho-Ser645) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of PKCd only when phosphorylated at serine 645.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 645 (R-L-S(p)-Y-S) derived from Human PKCd.
Target NamePKCd
ConjugateUnconjugated
Other NamesKPCD; PKC-delta; PRKCD; kinase PKC-delta; nPKC-delta
Accession NoSwiss-Prot: Q05655
NCBI Protein: NP_006245.2
Uniprot
Q05655
Gene ID
5580;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 78kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCd(Phospho-Ser645) Antibody #11296.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCd(Phospho-Ser645) Antibody #11296(left) or the same antibody preincubated with blocking peptide(right).
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
Kei Sakamoto, et,al. (2003) Am J Physiol Endocrinol Metab ; 285: E1081 - E1088.
Ling Zhang, et,al. (2004) J. Biol. Chem ; 279: 28315 - 28319.
Kristof Van Kolen et,al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et,al. (2002) J. Cell Biol ; 157: 253.
If you have published an article using product 11296, please notify us so that we can cite your literature.
et al,Protein kinase C mRNA and protein expressions in hypobaric hypoxia-induced cardiac hypertrophy in rats.
, (2010),
PMID:
19995357
et al,Protein kinase C mRNA and protein expressions in hypobaric hypoxia鈥恑nduced cardiac hypertrophy in rats.In Acta Physiol (Oxf) on 2010 Apr by M Uenoyama, S Ogata,et al..PMID:19995357
, (2010),
PMID:
19995357
et al,PKC 胃-Mediated Ca 2+/NF-AT Signalling Pathway May Be Involved in T-cell Immunosuppression in Coal-Burning Arsenic-Poisoned Population.In Environ Toxicol Pharmacol on 2017 Oct by Qibing Zeng , Peng Luo,et al..PMID: 28823652
, (2017),
PMID:
28823652
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