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Histone H3 (Di-Methyl-Lys27) Antibody #11583

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Product Detail

Product NameHistone H3 (Di-Methyl-Lys27) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around Di-Methylation site of lysine 27(A-R-K(di-methyl)-S-A) derived from Human Histone H3.

Target NameHistone H3

ConjugateUnconjugated

Other NamesH3/a, H3/c, H3/d, H3/f, H3/h

Accession NoSwiss-Prot#:P68431 NCBI Gene#:8351 NCBI Protein#:NP_003521.2

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Sdspage MW17KD

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C/1 year

Application Details

Predicted MW: 17kd

Western Blot: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from C6 and Hela cells using Histone H3 (Di-Methyl-Lys27) Antibody #11583.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3 (Di-Methyl-Lys27) Antibody #11583.

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

If you have published an article using product 11583, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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