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PEK/PERK (Phospho-Thr982) Antibody#11751

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Product Detail

Product NamePEK/PERK (Phospho-Thr982) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC

Species ReactivityHu

SpecificityThe antibody detects endogenous levels of PEK/PERK only when phosphorylated at threonine 982.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 982(R-H-T(p)-G-Q) derived from Human PEK/PERK.

Target NamePEK/PERK

ConjugateUnconjugated

Other Names E2AK3; PERK; PEK; EIF2AK3;

Accession NoSwiss-Prot#: Q9NZJ5;
NCBI Gene#: 9451;
NCBI Protein#: NP_004827.4.

Uniprot Q9NZJ5

Gene ID 9451;

Sdspage MW125kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr982) antibody #11751 (left)or the same antibody preincubated with blocking peptide (right).

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1)

Shi Y., An J., J. Biol. Chem. 274:5723-5730(1999).
Sood R., Biochem. J. 346:281-293(2000).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

If you have published an article using product 11751, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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