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Location： Home > Mouse Monoclonal Antibodies > Histone H2A.X(Phospho Ser139) Mouse mAb

Histone H2A.X(Phospho Ser139) Mouse mAb#12162

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Product NameHistone H2A.X(Phospho Ser139) Mouse mAb

Clone No.MR4523

Host SpeciesMouse

ClonalityMonoclonal

PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,IHC,IF,FC

Species ReactivityHuman,Mouse

SpecificityThis antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.

Immunogen DescSynthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.

Target NameH2AFX

Other NamesH2A histone family, member X ; H2A.X ; H2a/x

Uniprot P16104

Gene ID 3014

Calculated MW15KD

Sdspage MW15KD

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB 1:1000-1:2000
IHC 1:200-400
ICC 1:400
IF 1:50-200
All lanes: Histone H2A.X(Phospho Ser139) Mouse mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : 293 treated with UV for 15 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for Histone H2A.X(Phospho Ser139) using 12162 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence Histone H2A.X(Phospho Ser139) antibody (12162)

ICC/IF staining of Histone H2A.X(Phospho Ser139) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 12162 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti mouse, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control.
Nuclei were counterstained with DAPI.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

If you have published an article using product 12162, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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