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AKT1/2 (phospho-Thr308/309) Antibody#13311

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Product NameAKT1/2 (phospho-Thr308/309) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was purified by immunogen affinity chromatography.

ApplicationsWB

Species ReactivityHu,Ms,Rt

SpecificityRecognizes endogenous levels of AKT1/2 (phospho-Thr308/309) protein.

Immunogen DescKLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT1/2.

Target NameAKT1; AKT2

ConjugateUnconjugated

Other NamesPKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Accession NoSwiss-Prot#:P31749; P31751
NCBI Gene ID:207; 208

Uniprot P31749

Gene ID 207;

Calculated MW60KD

Concentration1 mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting:1:500 - 1:1000
Western blot analysis of AKT1/2 (phospho-Thr308/309) expression in HeLa, PC3 whole cell lysates.

If you have published an article using product 13311, please notify us so that we can cite your literature.

et al,DHCR24 Promotes Melanoma Stem-Like Cells Formation and Mediates Vemurafenib Resistance by Accumulating 27-Hydroxycholesterol , (2021),
PMID:
et al,Cholesterol neutralized vemurafenib treatment by promoting melanoma stem-like cells via its metabolite 27-hydroxycholesterol. In Cell Mol Life Sci on 2024 May 22 by Xiaohong Wang, Feiliang Zhong,et al..PMID:38775844 , (2024),
PMID: 38775844
et al,7?dehydrocholesterol suppresses melanoma cell proliferation and invasion via Akt1/NF?kB signaling,In Oncol Lett on 2020 Dec by Jia Liu, Feiliang Zhong, et al..PMID: 33193858 , (2020),
PMID: 33193858
et al,NVD-BM-mediated genetic biosensor triggers accumulation of 7-dehydrocholesterol and inhibits melanoma via Akt1/NF-?B signaling. In Aging (Albany NY) on 2020 Jul 25 by Jia Liu, Lei Cao, et al..PMID: 32712598 , (2020),
PMID: 32712598

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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