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Location： Home > Phospho Antibodies > Histone H3(Phospho-Ser10) Rabbit mAb

Histone H3(Phospho-Ser10) Rabbit mAb#13337

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Product NameHistone H3(Phospho-Ser10) Rabbit mAb

Clone No.SA31-01

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser10 of human Histone H3.

ConjugateUnconjugated

Other NamesH3 3 like sequence MH921 antibody
H3 3A antibody
H3 a antibody
H3 b antibody
H3 c antibody
H3 d antibody
H3 f antibody
H3 h antibody
H3 histone family member E pseudogene antibody
H3 i antibody
H3 j antibody
H3 k antibody
H3 l antibody
H33_HUMAN antibody
H3F3 antibody
H3f3b antibody
Histone H3 3 pseudogene antibody
Histone H3.3 antibody

Accession NoSwiss-Prot#:P68431

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Calculated MWPredicted band size: 15 kDa

Sdspage MWObserved band size: 17 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : Histone H3(Phospho-Ser10) Rabbit mAb at 1/1k dilution
Lane 1 : HeLa whole cell lysates
Lane 2 : HeLa treated with 100nM calyculin A for 30min whole cell lysates
Lane 3 : HeLa treated with calyculin A and alkaline phosphatase whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa

Exposure time: 6 seconds
All lanes : Histone H3(Phospho-Ser10) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa

Exposure time: 5 seconds
Formalin-fixed, paraffin-embedded human testis tissue stained for Histone H3 (Phospho-S10) using 13337 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for Histone H3 (Phospho-S10) using 13337 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Histone H3 (Phospho-S10) antibody (13337)

ICC/IF staining of Histone H3(Phospho-S10) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13337 at a working dilution of 1/100. The secondary antibody was Alexa Fluor®647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3. The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro.

If you have published an article using product 13337, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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