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Location： Home > Phospho Antibodies > c-Myc(Phospho-S62) Rabbit mAb

c-Myc(Phospho-S62) Rabbit mAb#13375

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Product Detail

Product Namec-Myc(Phospho-S62) Rabbit mAb

Clone No.ST49-08

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser62 of human c-Myc.

ConjugateUnconjugated

Other NamesAU016757 antibody
Avian myelocytomatosis viral oncogene homolog antibody
bHLHe39 antibody
c Myc antibody
Class E basic helix-loop-helix protein 39 antibody
MRTL antibody
Myc antibody
Myc protein antibody
Myc proto oncogene protein antibody
Myc proto-oncogene protein antibody
myc-related translation/localization regulatory factor antibody
MYC_HUMAN antibody
Myc2 antibody
MYCC antibody
Myelocytomatosis oncogene antibody
Niard antibody
Nird antibody
Oncogene Myc antibody
OTTHUMP00000158589 antibody
Proto-oncogene c-Myc antibody
Protooncogene homologous to myelocytomatosis virus antibody
RNCMYC antibody
Transcription factor p64 antibody
Transcriptional regulator Myc-A antibody
V-Myc avian myelocytomatosis viral oncogene homolog antibody
v-myc myelocytomatosis viral oncogene homolog (avian) antibody

Accession NoSwiss-Prot#:P01106

Uniprot P01106

Gene ID 4609;

Calculated MWPredicted band size: 50 kDa

Sdspage MWObserved band size: 57 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : c-Myc(Phospho-S62) Rabbit mAb at 1/1k dilution

Lane 1 : HCT116 whole cell lysates
Lane 2 : HCT116 treated with 200 nM PMA for 10 minutes whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 57 kDa

Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human lung tissue stained for c-Myc(Phospho-S62) using 13375 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for c-Myc(Phospho-S62) using 13375 at 1/100 dilution in immunohistochemical analysis.The picture on the right is blocked with the phospho peptide.
Immunocytochemistry/ Immunofluorescence cMyc(Phospho-S62) antibody (13375)

ICC/IF staining of cMyc(Phospho-S62) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13375 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

If you have published an article using product 13375, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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