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Tau(Phospho-S396) Rabbit mAb#13400

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Product Detail

Product NameTau(Phospho-S396) Rabbit mAb

Clone No.SN62-09

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescA synthesized peptide derived from human Tau around the phosphorylation site of S396

ConjugateUnconjugated

Other NamesAI413597 antibody
AW045860 antibody
DDPAC antibody
FLJ31424 antibody
FTDP 17 antibody
G protein beta1/gamma2 subunit interacting factor 1 antibody
MAPT antibody
MAPTL antibody
MGC134287 antibody
MGC138549 antibody
MGC156663 antibody
Microtubule associated protein tau antibody
Microtubule associated protein tau isoform 4 antibody
Microtubule-associated protein tau antibody
MSTD antibody
Mtapt antibody
MTBT1 antibody
MTBT2 antibody
Neurofibrillary tangle protein antibody
Paired helical filament tau antibody
Paired helical filament-tau antibody
PHF tau antibody
PHF-tau antibody
PPND antibody
PPP1R103 antibody
Protein phosphatase 1, regulatory subunit 103 antibody
pTau antibody
RNPTAU antibody
TAU antibody
TAU_HUMAN antibody
Tauopathy and respiratory failure, included antibody

Accession NoSwiss-Prot#:P10636

Uniprot P10636

Gene ID 4137;

Calculated MWPredicted band size: 79 kDa

Sdspage MWObserved band size: 50-80 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes :Tau(Phospho-S396) Rabbit mAb at 1/500 dilution

Lane 1 : Mouse brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 50-80 kDa

Exposure time: 12 seconds
All lanes :Tau(Phospho-S396) Rabbit mAb at 1/500 dilution

Lane 1 : SH-SY5Y treated with AP whole cell lysates
Lane 2 : SH-SY5Y whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 50-80 kDa

Exposure time: 12 seconds
All lanes :Tau(Phospho-S396) Rabbit mAb at 1/500 dilution

Lane 1 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 50-80 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Tau(Phospho-S396) using 13400 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Tau (Phospho-S396) antibody (13400)

ICC/IF staining of Tau(Phospho-S396) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13400 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both.

If you have published an article using product 13400, please notify us so that we can cite your literature.

et al,Genetic deletion of 伪7 nicotinic acetylcholine receptors induces an age-dependent Alzheimer鈥檚 disease-like pathology. In Prog Neurobiol on 2021 Nov by Maria Rosaria Tropea, Domenica D Li Puma,et al..PMID:34453977 , (2021),
PMID: 34453977
et al,Effectiveness and mechanisms of combined use of antioxidant nutrients in protecting against oxidative stress-induced neuronal loss and related neurological deficits. In CNS Neurosci Ther on 2024 Jul by Lu Wang, Yingjuan Wei,et al..PMID:39072940 , (2024),
PMID: 39072940

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;