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Location： Home > Phospho Antibodies > PKC zeta(Phospho-T560) Rabbit mAb

PKC zeta(Phospho-T560) Rabbit mAb#13403

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Product Detail

Product NamePKC zeta(Phospho-T560) Rabbit mAb

Clone No.SN0753

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr560 of human PKC zeta.

ConjugateUnconjugated

Other Names14-3-3-zetaisoform antibody
AI098070 antibody
aPKCzeta antibody
C80388 antibody
EC 2.7.11.13 antibody
KPCZ_HUMAN antibody
nPKC zeta antibody
nPKC-zeta antibody
OTTHUMP00000001368 antibody
OTTHUMP00000044160 antibody
PKC 2 antibody
PKC ZETA antibody
PKC2 antibody
Pkcz antibody
PKCZETA antibody
PKM-zeta, included antibody
PRKCZ antibody
Protein kinase C zeta antibody
Protein kinase C zeta form antibody
Protein kinase C zeta type antibody
r14-3-3 antibody
R74924 antibody
zetaPKC antibody

Accession NoSwiss-Prot#:Q05513

Uniprot Q05513

Gene ID 5590;

Calculated MWPredicted band size: 68 kDa

Sdspage MWObserved band size: 68 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : PKC zeta(Phospho-T560) Rabbit mAb at 1/1k dilution

Lane 1 : C6 whole cell lysates
Lane 2 : C6 treated with 200nM Calyculin A for 1 hours whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PKC zeta(Phospho-T560) using 13403 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse brain tissue stained for PKC zeta(Phospho-T560) using 13403 at 1/100 dilution in immunohistochemical analysis.
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, , h, θ, λ/ι, ? and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

If you have published an article using product 13403, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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