Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > KD/KO Validation > DNA PKcs (Phospho-Ser2056) Rabbit mAb

DNA PKcs (Phospho-Ser2056) Rabbit mAb#14139

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameDNA PKcs (Phospho-Ser2056) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman

SpecificityPhospho-DNA PKcs (S2056) Antibody detects endogenous levels of total Phospho-DNA PKcs (S2056)

Immunogen DescA synthesized peptide derived from human Phospho-DNA PKcs (S2056)

ConjugateUnconjugated

Other NamesDNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA-PK

Accession NoUniprot:P78527

Uniprot P78527

Calculated MW469kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IHC:1:50~1:200
ICC/IF:1:50~1:200

All lanes: DNA PKcs (Phospho-Ser2056) Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lane 2 : JK treated with alkaline phosphatase whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 469 kDa
Observed band size: 469 kDa

Exposure time: 12 seconds
All lanes : DNA PKcs (Phospho-Ser2056) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : DNA PKcs (Phospho-Ser2056) knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon tissue stained for DNA PKcs (Phospho-Ser2056) using 14139 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 14139, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors