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KD/KO Validation > AMPK alpha 1 (Phospho-Ser496) Rabbit mAb
AMPK alpha 1 (Phospho-Ser496) Rabbit mAb#14215
Yes
All lanes: AMPK alpha 1 (Phospho-Ser496) Rabbit mAb at 1/1k dilution
Lane 1 : 293T treated with alkaline phosphatase whole cell lysates
Lane 2 : 293T whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 4 seconds
All lanes:AMPK alpha 1 (Phospho-Ser496)Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : AMPK alpha 1 (Phospho-Ser496) knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence AMPK alpha 1 (Phospho-Ser496) antibody (14215) ICC/IF staining of AMPK alpha 1 (Phospho-Ser496) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14215 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
